pe miltenyi cat 130 120 698 at manufacturer s recommended concentration for 15 min followed by pbs washing centrifugation repeat as before  (Miltenyi Biotec)

Journal: iScience

Article Title: Broad anti-sarbecovirus responses elicited by a single administration of mosaic-8 RBD-nanoparticle vaccine prepared using atomic layer deposition

doi: 10.1016/j.isci.2025.113649

Figure Lengend Snippet: ALD-coated mosaic-8b immunization elicits an IgG1-dominated response Analysis of binding interactions of IgG1, IgG2a, IgG2b, IgG3, FcγR2b-binding IgGs, FcγR3-binding IgGs, FcγR4-binding IgGs, and total IgG with the indicated spikes, RBDs, or a non-sarbecovirus control protein. Antigen names (y axes) are colored to identify clades from which spikes or RBDs were derived. Matched antigens are indicated with gray shading around the name, mismatched antigens are indicated with a black outline around the name, and WA1 antigens that are matched to the WA1 pre-vaccination are indicated with a dashed black outline around the name. (A and B) Serum from mice immunized with ALD mix3, ALD mix4, SD, and conventional mosaic-8b (x axes) in originally naive (A) or pre-vaccinated (B) cohorts was tested for binding against different sarbecovirus spikes and RBDs (y axes). Each vertical line of binding data represents an individual mouse, and immunization groups are separated by a vertical black line. Unimmunized = Day 0 serum from a naive mouse prior to mosaic-8b immunization. MFI = median fluorescent intensity.

Article Snippet: Goat Anti-Mouse IgG2b, Human ads-PE , Southern Biotech , Cat # 1090-09S; RRID: AB_2794524.

Techniques: Binding Assay, Control, Derivative Assay

Journal: iScience

Article Title: Broad anti-sarbecovirus responses elicited by a single administration of mosaic-8 RBD-nanoparticle vaccine prepared using atomic layer deposition

doi: 10.1016/j.isci.2025.113649

Figure Lengend Snippet: ALD-coated mosaic-8b immunization elicits different IgG subclass and FcγR responses than conventional mosaic-8b immunizations (A and B) For IgG1, IgG2a, IgG2b, IgG3, FcγR2b-binding IgGs, FcγR3-binding IgGs, FcγR4-binding IgGs, and total IgG, geomean MFI (geometric median fluorescent intensity) values from the naive cohort (A) or the pre-vaccinated cohort (B) are represented as points corresponding to different spikes or RBDs (individual responses shown in ) and compared pairwise across immunization cohorts by Tukey’s multiple comparison test calculated by GraphPad Prism. Data for each immunization group are visualized using box and whisker plots. The boxes display the range between the upper and lower quartiles, with a line denoting the median value. The whiskers extend to the minimum and maximum values, excluding any outliers. Significant differences between cohorts linked by vertical lines in panels B and C are indicated by asterisks: p < 0.05 = ∗, p < 0.01 = ∗∗, p < 0.001 = ∗∗∗, p < 0.0001 = ∗∗∗∗.

Article Snippet: Goat Anti-Mouse IgG2b, Human ads-PE , Southern Biotech , Cat # 1090-09S; RRID: AB_2794524.

Techniques: Binding Assay, Comparison, Whisker Assay